ISSN 0004-881X

Development of Biocompatible Apatite Sheets with Various Ca/P Ratios and Carbonate Ion Contents for Mouse Osteoblastic Cell Culture and their Evaluations

by Ueno H. ¹, Fujimi T.J. ², Okada I. ¹, Aizawa M. ^2*

1) Department of Chemistry, Faculty of Science and Engineering, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo, 102-8554, Japan
2) Department of Applied Chemistry, School of Science and Technology, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, 214-8571, Japan

Abstract

Fibrous hydroxyapatite (f-HAp) particles were synthesized by a homogeneous precipitation method. The molar ratio of calcium per phosphorus (Ca/P) of f-HAp could be controlled by using the starting solutions with varying Ca/P ratios. Porous sheets and dense ceramics for cell culture were fabricated from the synthesized apatite fibers. Carbonate ion contents of the fired samples were controlled by carbon dioxide content in the firing atmosphere. Mouse osteoblastic cells were cultured on the samples to assess cellular activities for the varying compositions and structures. The sample with higher carbonate ion content had better results in cell proliferation and alkaline phosphatase activity. The same tendency for the cellular responses was observed in the samples with lower Ca/P ratios. These results indicate that the materials from f-HAp with higher carbonate ion contents and lower Ca/P ratios, which are closer to the chemical compositions of natural bone, are suited for osteoblastic cell culture.

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